Interplay Between Oxytetracycline and the Homozygote kdr (L1014F) Resistance Genotype on Fecundity in Anopheles gambiae (Diptera: Culicidae) Mosquitoes

Abstract The insecticide resistance in Anopheles gambiae mosquitoes has remained the major threat for vector control programs but fitness effects conferred by these mechanisms are poorly understood. To fill this knowledge gap, present study aimed at testing hypothesis that antibiotic oxytetracycline could have an interaction with genotypes and consequently inhibit fecundity An. gambiae. Four strains of gambiae: Kisumu (susceptible), KisKdr (kdr (L1014F) resistant), AcerKis (ace-1 (G119S) resistant) AcerKdrKis (both kdr ace-1 were used study. different allowed to bloodfeed on a rabbit previously treated concentration 39·10–5 M. Three days later, ovarian follicles dissected from individual mosquito ovaries into physiological saline solution (0.9% NaCl) under stereomicroscope eggs counted. Fecundity was substantially lower oxytetracycline-exposed females when compared untreated individuals females. exposed displayed increased their nontreated counterparts whereas they had reduced There no substantial difference between Our data indicate indirect effect reducing carrying kdrR genotype. These findings be useful designing new integrated approaches malaria endemic countries.